WebRNA-Seq libraries were prepared following the Illumina the TruSeq Stranded mRNASamplePreparationKit(CatalognumbersRS 122 2101orRS 122 2102). Spike-inRNAsareobtainedfromNIH(CatalognumberERCC 78A 11119). ... 1.Dilute 1.0 g of the total RNA with nuclease-free ultra pure water to a finalvolumeof25 … WebApr 12, 2024 · Total RNA was extracted from muscle in 6-week-old Tarsl2 +/+ and Tarsl2-/-mouse and isolated using TRIzol reagent. The RNA-seq libraries were prepared using …
TruSeq Stranded Total RNA with Ribo-Zero Plant For plant ...
Webu Human—UniversalHumanReference(UHR)RNA u Mouse—UniversalMouseReferenceRNA u Rat—UniversalRatReferenceRNA u Bacteria—Escherichiacoli(Gram-)orBacillussubtilis(Gram+) ... Instructions for preparing libraries using the TruSeq Stranded Total RNA library prep with Ribo-Zero Plus rRNA Depletion. ... WebA simple, extensible, cost-effective, rapid single-day solution for evaluating the coding transcriptome leveraging as slight as 25 ng input of standard (non-degraded) RNA. henry cardenas music
Illumina TruSeq Stranded total Sample Preparation Protocol
WebApr 11, 2024 · Technologies) was used to quantify and allow for 200 ng of RNA to be used for each sample. RNA was poly(A) selected and reverse transcribed using a TruSeq Stranded mRNA Library Preparation Kit (Illumina). Each sample was barcoded (8-base) for multiplex analysis and amplified (15 cycle PCR). Each library was sequenced on a HiSeq … WebRapid stranded total RNA-Seq prep supports a broad range of sample types for studying the coding and non-coding transcriptome providing unparalleled study flexibility. Kit contains … WebAug 25, 2024 · The terms may refer to nucleic acids of any compositional form, such as deoxyribonucleic acid (DNA, e.g., complementary DNA (cDNA), genomic DNA (gDNA) and the like), ribonucleic acid (RNA, e.g., message RNA (mRNA), short inhibitory RNA (siRNA), ribosomal RNA (rRNA), transfer RNA (tRNA), microRNA, RNA highly expressed by the … henry carbine 357