WebJun 1, 2024 · In fish, irf7 has been identified in more than twenty species, and their functions of inducing antiviral factors have also been confirmed (Cui et al., 2011; Holland et al., ... qPCR analysis. For qPCR analysis, EPC cells were seeded in 6-well plates overnight, and transfected with 2000 ng corresponding expression plasmid. ... WebInterferon regulatory factors (irfs) are a family of genes that encode transcription factors with important roles in regulating the expression of Type I interferons (IFNs) and other genes associated with related pathways. irfs have multitudinous functions in growth, development and regulation of onc …
Irf7 - PrimePCR Assay and Template Life Science Bio-Rad
WebNov 23, 2024 · We further demonstrated that inflammasome activation and IL-1 signaling regulated type I IFN signaling and SOCS1 expression through a MyD88-TRAF3-IRF3-dependent pathway, which inhibits... WebMar 14, 2024 · Quantitative PCR (qPCR) was performed as previously described [ 16 ]. Total RNA was extracted from cells by using an RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. The... hilary ryan actress
STING-mediated type-I interferons contribute to the …
WebDec 1, 2024 · For quantitative analysis of RSIV DNA by real-time qPCR, the method of Caipang et al. (2003) was used. PCR was performed using a Thermal Cycler Dice® Real Time System Ⅲ (Takara). 3. Results ... IRF3 and IRF7 of the IRF family are well documented to play an important role in inducing the expression of the type I IFN gene in virus-infected ... WebJul 14, 2016 · IRF3-5D, an active form of IRF3, and IRF7Δ247–467, a constitutively active form of IRF7 were constructed as previously described using pFLAG-IRF3, and pFLAG-IRF7 plasmids as templates 38, 39,... WebJun 1, 2024 · Human interferon regulatory factor 7 (IRF7) plays an important role in the innate antiviral immune response. To date, the characteristics and functions of waterfowl IRF7 have not been... small-world networks pdf