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Western Blot Protocols and Recipes - Thermo Fisher Scientific

WebAcrylamide/Bis-acrylamide, 30% solution BioReagent, suitable for electrophoresis, 37.5:1; Synonyms: 30% Acrylamide/Bisacrylamide 37.5:1,Polyacrylamide solution; find Sigma-Aldrich-A3699 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Web1:5,000 (0.01–0.2 µg/mL) 1:5,000 (0.2–1.0 µg/mL) Incubate the membrane protein-side up in the primary antibody solution with agitation, for 1 hour at room temperature or overnight at 2–8°C. Ensure the volume of the antibody solution is enough to fully cover the membrane. Wash the membrane 3 times with agitation for 10 minutes each in ... WebAcrylamide/N,N'-methylenebisacrylamide (29:1), 79-06-1, 110-26-9, is an acrylamide formulation used for separation of biomolecules. Learn more at Thermo Fisher Scientific. how many times is melchizedek listed in bible