WebDec 26, 2024 · Results: We knocked down CTCF in EL4 cells using shRNA, and conducted single cell RNA-seq on both wild type (WT) cells and CTCF-Knockdown (CTCF-KD) cells using Fluidigm C1 system. Principal component analysis of single cell RNA-seq data showed that WT and CTCF-KD cells concentrated in two different clusters on PC1, … WebNov 5, 2012 · Mutation of CTCF-binding sites in plasmid-borne promoters reduced transcriptional activity in an orientation-dependent manner. Depletion of CTCF by …
NSD2 E1099K drives relapse in pediatric acute lymphoblastic …
WebCTCF (the CCCTC-binding factor) is a sequence-specific DNA binding protein that recognizes extended DNA motifs with its 11 zinc fingers and acts as a critical regulator of interphase genome folding (Splinter et al ... (NT) shRNA. (G) Effects of 2 h of LPS stimulation on the 7543 H3K9me3 blocks methylated by SETDB1. (H) ... WebSep 1, 2016 · Our results explain how epigenetic factor CTCF-mediated chromatin remodeling regulates interactions between eye-specific PAX6 and those genes that are induced/associated with cell differentiation to modulate corneal epithelial cell-specific differentiation. ... Diminished CTCF mRNA with CTCF-shRNA in HTCE cells weakened … curlsbot test
Role of CCCTC binding factor (CTCF) and cohesin in the
WebNov 30, 2024 · Inhibition of cell proliferation and clonogenicity following CTCF shRNA knockdown in K562 cells. (A) Immunoblot of CTCF shRNA knockdown in the presence and absence of doxycycline (dox) compared to ... WebUsing 3C-based experiments in B cells where CTCF protein levels were downregulated by shRNA-mediated CTCF depletion or Mb1-Cre-mediated conditional deletion of the Ctcf gene in vivo, we and others have shown that in the absence of CTCF IgH locus contraction is only modestly affected (Degner et al., 2009) and Igκ long-range chromatin ... WebSep 19, 2024 · The CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF acetylation … curls book