WebNov 5, 2014 · The way that I typically incorporate cisplatin viability staining into my live cell staining protocols is to perform my antibody staining (in 100uL FACS buffer), then add 100uL of 2X cisplatin (diluted 1:500 from Fluidigm stock in PBS) and incubate for 5 minutes RT, then wash and fix. This reduces wash steps, which minimizes cell loss and seems ... WebWe support the scientific community in the quest to solve unanswered challenges in health and disease across basic, translational and clinical research, including the fields of cancer, inflammatory diseases, immunotherapies and environmental monitoring. Harnessing the power of proprietary CyTOF® and microfluidics technologies, Fluidigm products provide …
CyTOForum • View topic - cisplatin 198 labelling
WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … Web$246.00 / Case of 2500 Description Potent pro-apoptotic anticancer agent; activates caspase-3 Potent anticancer agent that blocks DNA synthesis. Induces apoptosis via p53-dependent and -independent mechanisms. Inhibits X-linked inhibitor of apoptosis protein (XIAP) expression and activates caspase-3. how does adhd affect your life
Cisplatin - an overview ScienceDirect Topics
WebJul 25, 2024 · The first platinum anticancer drug cisplatin is widely used to treat various types of cancers including ovarian, testicular, bladder, colorectal, lung, and head and neck cancers [ 16, 17 ]. Cisplatin crosslinks with purine bases on the DNA, interfering with DNA repair mechanisms, causing DNA damage and inducing apoptosis [ 16 ]. WebCisplatin quickly and freely diffuses into dead cells with compromised membranes and forms covalent sulfhydryl bonds with intracellular protein nucleophiles. Cisplatin is … WebFeb 3, 2024 · Imaging Mass Cytometry (IMC) is an expansion of mass cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light … phosphoric acid vs phosphonic acid